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Fluorescence Filters
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Datasheet (PDF)
Lightwaves2020’s fluorescence filters are specially designed
and manufactured for cofocal microscopic imaging applications. With
state-of-the-art optical coating facility, coupled with its advanced
proprietary coating technology, Lightwaves2020’s fluorescence
filters have low auto-fluorescence, high transmittance and high blocking,
high imaging contrast as well as high durability and reliability.
We provide standard fluorescence filter sets and custom design with manufacturing
for special applications.
 Features
- High transmission: > 90%
- High reflection (dichroic beam splitter): > 98%;
- Steep slope
- Hard dielectric coating with superior environmental reliability and
durability
Typical Fluorescence Filter Sets
| Fluorophore |
Exciter |
Emistter |
Dichroic |
| CWL (nm) |
BW (nm) |
CWL (nm) |
BW (nm) |
Reflection (nm) |
| CFP |
438 |
20 |
483 |
30 |
426-450 |
| CY3 |
531 |
30 |
593 |
75 |
499-555 |
| CY5 |
628 |
60 |
692 |
70 |
594-651 |
| DAPI |
377 |
50 |
447 |
50 |
344-404 |
| FITC |
482 |
30 |
536 |
40 |
466-500 |
| GFP |
472 |
40 |
520 |
50 |
442-488 |
| Texas Red |
562 |
40 |
624 |
60 |
530-585 |
| TRITC |
543 |
50 |
593 |
55 |
499-555 |
| YFP |
500 |
20 |
542 |
30 |
488-513 |
Sample Spectrum
Excellent
Uniformity
As shown below, we are able to make those fluorescence filters
with very good uniformity. For instance, the center wavelength variation
over a 3”x3” wafer is less than 0.5nm.
This will result in much better image, especially in a system with large
NA and large beam size.
High
Transmission
Our fluorescence filters have high transmission, with transmittance
higher than 85% in UV, and larger than 90% in visible.
(See spectra below, measured at wafer level before AR is applied on the
back).
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